px458 vector Search Results


px458/ notch1 sgrna  (Lonza)
90
Lonza px458/ notch1 sgrna
<t>NOTCH1</t> was targeted with CRISPR/Cas9. A, Structure of CRISPR/Cas9 and sgRNA plasmid. B, NOTCH1 protein structure and truncated NOTCH1 after targeting by CRISPR/Cas9 and sgRNA. C, Morphology of wild type (WT) and truncated NOTCH1 (NOTCH1−/−) iPSCs. Scale bars represent 200 μm. D, Morphology of NCSCs with WT and NOTCH1−/− that were differentiated from iPSCs counterparts. Scale bars represent 500 μm. E, Left panel: Western blot of NOTCH1, OCT4, and GAPDH in iPSCs and NCSCs with WT and NOTCH1−/−. Middle and right panels: quantification of Western blot analysis. The signal was normalized to GAPDH and then normalized to WT iPSCs. In the middle panel, **P<.01, NOTCH1−/− iPSCs versus WT iPSCs; and NOTCH1−/− NCSCs versus WT NCSCs separately. In the right panel, **P<.01, WT NCSCs versus WT iPSCs; and NOTCH1−/− NCSC versus NOTCH−/− iPSCs separately. Experiments were repeated 3 times and all data points and median were plotted. U6, U6 promoter; sgRNA, single-guide RNA; CBh, CBh promoter; 2A GFP, 2A green fluorescent protein; EGF, epidermal growth factor; LNR, LIN-12/notch repeat motif; TM, transmembrance domain; ANK, ankyrin repeat; PEST, PEST domain; iPSCs, induced pluripotent stem cells; NCSCs, neural crest stem cells; OCT4, octamer-binding transcription factor 4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Px458/ Notch1 Sgrna, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/px458/ notch1 sgrna/product/Lonza
Average 90 stars, based on 1 article reviews
px458/ notch1 sgrna - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
px458  (Polysciences inc)
90
Polysciences inc px458
<t>NOTCH1</t> was targeted with CRISPR/Cas9. A, Structure of CRISPR/Cas9 and sgRNA plasmid. B, NOTCH1 protein structure and truncated NOTCH1 after targeting by CRISPR/Cas9 and sgRNA. C, Morphology of wild type (WT) and truncated NOTCH1 (NOTCH1−/−) iPSCs. Scale bars represent 200 μm. D, Morphology of NCSCs with WT and NOTCH1−/− that were differentiated from iPSCs counterparts. Scale bars represent 500 μm. E, Left panel: Western blot of NOTCH1, OCT4, and GAPDH in iPSCs and NCSCs with WT and NOTCH1−/−. Middle and right panels: quantification of Western blot analysis. The signal was normalized to GAPDH and then normalized to WT iPSCs. In the middle panel, **P<.01, NOTCH1−/− iPSCs versus WT iPSCs; and NOTCH1−/− NCSCs versus WT NCSCs separately. In the right panel, **P<.01, WT NCSCs versus WT iPSCs; and NOTCH1−/− NCSC versus NOTCH−/− iPSCs separately. Experiments were repeated 3 times and all data points and median were plotted. U6, U6 promoter; sgRNA, single-guide RNA; CBh, CBh promoter; 2A GFP, 2A green fluorescent protein; EGF, epidermal growth factor; LNR, LIN-12/notch repeat motif; TM, transmembrance domain; ANK, ankyrin repeat; PEST, PEST domain; iPSCs, induced pluripotent stem cells; NCSCs, neural crest stem cells; OCT4, octamer-binding transcription factor 4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Px458, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/px458/product/Polysciences inc
Average 90 stars, based on 1 article reviews
px458 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


NOTCH1 was targeted with CRISPR/Cas9. A, Structure of CRISPR/Cas9 and sgRNA plasmid. B, NOTCH1 protein structure and truncated NOTCH1 after targeting by CRISPR/Cas9 and sgRNA. C, Morphology of wild type (WT) and truncated NOTCH1 (NOTCH1−/−) iPSCs. Scale bars represent 200 μm. D, Morphology of NCSCs with WT and NOTCH1−/− that were differentiated from iPSCs counterparts. Scale bars represent 500 μm. E, Left panel: Western blot of NOTCH1, OCT4, and GAPDH in iPSCs and NCSCs with WT and NOTCH1−/−. Middle and right panels: quantification of Western blot analysis. The signal was normalized to GAPDH and then normalized to WT iPSCs. In the middle panel, **P<.01, NOTCH1−/− iPSCs versus WT iPSCs; and NOTCH1−/− NCSCs versus WT NCSCs separately. In the right panel, **P<.01, WT NCSCs versus WT iPSCs; and NOTCH1−/− NCSC versus NOTCH−/− iPSCs separately. Experiments were repeated 3 times and all data points and median were plotted. U6, U6 promoter; sgRNA, single-guide RNA; CBh, CBh promoter; 2A GFP, 2A green fluorescent protein; EGF, epidermal growth factor; LNR, LIN-12/notch repeat motif; TM, transmembrance domain; ANK, ankyrin repeat; PEST, PEST domain; iPSCs, induced pluripotent stem cells; NCSCs, neural crest stem cells; OCT4, octamer-binding transcription factor 4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Journal: The Journal of thoracic and cardiovascular surgery

Article Title: Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy

doi: 10.1016/j.jtcvs.2018.02.087

Figure Lengend Snippet: NOTCH1 was targeted with CRISPR/Cas9. A, Structure of CRISPR/Cas9 and sgRNA plasmid. B, NOTCH1 protein structure and truncated NOTCH1 after targeting by CRISPR/Cas9 and sgRNA. C, Morphology of wild type (WT) and truncated NOTCH1 (NOTCH1−/−) iPSCs. Scale bars represent 200 μm. D, Morphology of NCSCs with WT and NOTCH1−/− that were differentiated from iPSCs counterparts. Scale bars represent 500 μm. E, Left panel: Western blot of NOTCH1, OCT4, and GAPDH in iPSCs and NCSCs with WT and NOTCH1−/−. Middle and right panels: quantification of Western blot analysis. The signal was normalized to GAPDH and then normalized to WT iPSCs. In the middle panel, **P<.01, NOTCH1−/− iPSCs versus WT iPSCs; and NOTCH1−/− NCSCs versus WT NCSCs separately. In the right panel, **P<.01, WT NCSCs versus WT iPSCs; and NOTCH1−/− NCSC versus NOTCH−/− iPSCs separately. Experiments were repeated 3 times and all data points and median were plotted. U6, U6 promoter; sgRNA, single-guide RNA; CBh, CBh promoter; 2A GFP, 2A green fluorescent protein; EGF, epidermal growth factor; LNR, LIN-12/notch repeat motif; TM, transmembrance domain; ANK, ankyrin repeat; PEST, PEST domain; iPSCs, induced pluripotent stem cells; NCSCs, neural crest stem cells; OCT4, octamer-binding transcription factor 4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: 13 One million iPSCs were electrotransfected with constructed 5 μ g of PX458/ NOTCH1 sgRNA using Lonza Human Stem Cell Nucleofector Kit 2 with program U-023 on a Nuclefector 2 device (Lonza Ltd, Basel, Switzerland).

Techniques: CRISPR, Plasmid Preparation, Western Blot, Binding Assay

DNA sequencing result of NOTCH1 homozygous knockout (NOTCH1−/−) cell line. A, Wild type (WT) NOTCH1 sequence, gRNA target site and protospacer adjacent motif (PAM) position. B, Sanger DNA sequencing result of NOTCH1 homozygous (NOTCH1−/−) knockout cell line. The sequence of NOTCH1−/− gene is shown. The vertical black line indicates the position of the deletion of 7 base pairs (bp). The deletion of 7 bp in NOTCH1 results in a frameshift mutation at the 1108th amino acid and a premature stop codon at 1177. The resulting protein lacks NOTCH1 amino acids from residue 1108 to the C-terminus. gRNA, Guide RNA; hNOTCH1, human NOTCH1 gene.

Journal: The Journal of thoracic and cardiovascular surgery

Article Title: Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy

doi: 10.1016/j.jtcvs.2018.02.087

Figure Lengend Snippet: DNA sequencing result of NOTCH1 homozygous knockout (NOTCH1−/−) cell line. A, Wild type (WT) NOTCH1 sequence, gRNA target site and protospacer adjacent motif (PAM) position. B, Sanger DNA sequencing result of NOTCH1 homozygous (NOTCH1−/−) knockout cell line. The sequence of NOTCH1−/− gene is shown. The vertical black line indicates the position of the deletion of 7 base pairs (bp). The deletion of 7 bp in NOTCH1 results in a frameshift mutation at the 1108th amino acid and a premature stop codon at 1177. The resulting protein lacks NOTCH1 amino acids from residue 1108 to the C-terminus. gRNA, Guide RNA; hNOTCH1, human NOTCH1 gene.

Article Snippet: 13 One million iPSCs were electrotransfected with constructed 5 μ g of PX458/ NOTCH1 sgRNA using Lonza Human Stem Cell Nucleofector Kit 2 with program U-023 on a Nuclefector 2 device (Lonza Ltd, Basel, Switzerland).

Techniques: DNA Sequencing, Knock-Out, Sequencing, Mutagenesis, Residue

Cardiovascular progenitor cells (CVPCs) and endothelial cells (ECs) differentiation from iPSCs with wild type (WT) and truncated NOTCH1 (NOTCH1−/−). A, Quantitative polymerase chain reaction of ISL1, NKX2.5, and MYOCD expression level in CVPCs with WT and NOTCH1−/−. Expression was normalized with GAPDH and further normalized to WT CVPCs. Experiments were repeated 3 times and all data points and median were plotted. **P<.01, NOTCH1−/− CVPCs versus WT CVPCs. B, NOTCH1 immunostaining of WT CVPCs, which were differentiated from iPSC counterparts. Scale bars represent 100 μm. C, CD31 immunostaining of ECs that differentiated from CVPCs with WTand NOTCH1−/−. Scale bars represent 100 μm. D, Quantitative polymerase chain reaction of CD105 and CD31 expression level in ECs that differentiated from CVPCs with WT and NOTCH1−/−. Expression was normalized with GAPDH and further normalized to WT ECs. Experiments were repeated 3 times and all data points and median were plotted. **P<.01, NOTCH1−/− ECs versus WT ECs. ISL1, islet1; NKX2.5, NK2 homeobox 5; MYOCD, myocardin; DAPI, 4′,6-diamidino-2-phenylindole; CD105, cluster of differentiation 105; CD31, cluster of differentiation 31.

Journal: The Journal of thoracic and cardiovascular surgery

Article Title: Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy

doi: 10.1016/j.jtcvs.2018.02.087

Figure Lengend Snippet: Cardiovascular progenitor cells (CVPCs) and endothelial cells (ECs) differentiation from iPSCs with wild type (WT) and truncated NOTCH1 (NOTCH1−/−). A, Quantitative polymerase chain reaction of ISL1, NKX2.5, and MYOCD expression level in CVPCs with WT and NOTCH1−/−. Expression was normalized with GAPDH and further normalized to WT CVPCs. Experiments were repeated 3 times and all data points and median were plotted. **P<.01, NOTCH1−/− CVPCs versus WT CVPCs. B, NOTCH1 immunostaining of WT CVPCs, which were differentiated from iPSC counterparts. Scale bars represent 100 μm. C, CD31 immunostaining of ECs that differentiated from CVPCs with WTand NOTCH1−/−. Scale bars represent 100 μm. D, Quantitative polymerase chain reaction of CD105 and CD31 expression level in ECs that differentiated from CVPCs with WT and NOTCH1−/−. Expression was normalized with GAPDH and further normalized to WT ECs. Experiments were repeated 3 times and all data points and median were plotted. **P<.01, NOTCH1−/− ECs versus WT ECs. ISL1, islet1; NKX2.5, NK2 homeobox 5; MYOCD, myocardin; DAPI, 4′,6-diamidino-2-phenylindole; CD105, cluster of differentiation 105; CD31, cluster of differentiation 31.

Article Snippet: 13 One million iPSCs were electrotransfected with constructed 5 μ g of PX458/ NOTCH1 sgRNA using Lonza Human Stem Cell Nucleofector Kit 2 with program U-023 on a Nuclefector 2 device (Lonza Ltd, Basel, Switzerland).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Immunostaining

The NOTCH1 knockout shows severely impaired SMC differentiation. A, P75 immunostaining of NCSCs with wild type (WT) and truncated NOTCH1 (NOTCH1−/−) that were differentiated from iPSC counterparts. Scale bars represent 100 μm. B, Quantitative polymerase chain reaction of SOX10 and TFAP2A expression level in iPSCs and NCSCs with WT and NOTCH1−/−. Expression was normalized with GAPDH and further normalized to WT iPSCs. Experiments were repeated 3 times and all data points and median were plotted. **P<.01, NOTCH1−/− NCSCs versus WT NCSCs. C, SMA immunostaining of SMCs that differentiated from NCSCs with WT and NOTCH1−/−. Scale bars represent 100 μm. D, Western blot of expressions of MYH11, SMA, CNN1, TAGLN, and GAPDH in SMCs differentiated from NCSCs with WT and NOTCH1−/−. E, Quantification of Western blot analysis. The signal was normalized to GAPDH and then normalized to WT SMCs. *P<.05; **P<.01, NOTCH1−/− SMCs versus WT SMCs. DAPI, 4′,6-diamidino-2-phenylindole; SOX10, SRY-related HMG-box 10; iPSCs, induced pluripotent stem cells; NCSCs, neural crest stem cells; TFAP2A, transcription factor AP-2 alpha; SMA, smooth muscle α-actin; SMC, smooth muscle cell; MYH11, myosin 11; CNN1, calponin 1; TAGLN, transgelin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Journal: The Journal of thoracic and cardiovascular surgery

Article Title: Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy

doi: 10.1016/j.jtcvs.2018.02.087

Figure Lengend Snippet: The NOTCH1 knockout shows severely impaired SMC differentiation. A, P75 immunostaining of NCSCs with wild type (WT) and truncated NOTCH1 (NOTCH1−/−) that were differentiated from iPSC counterparts. Scale bars represent 100 μm. B, Quantitative polymerase chain reaction of SOX10 and TFAP2A expression level in iPSCs and NCSCs with WT and NOTCH1−/−. Expression was normalized with GAPDH and further normalized to WT iPSCs. Experiments were repeated 3 times and all data points and median were plotted. **P<.01, NOTCH1−/− NCSCs versus WT NCSCs. C, SMA immunostaining of SMCs that differentiated from NCSCs with WT and NOTCH1−/−. Scale bars represent 100 μm. D, Western blot of expressions of MYH11, SMA, CNN1, TAGLN, and GAPDH in SMCs differentiated from NCSCs with WT and NOTCH1−/−. E, Quantification of Western blot analysis. The signal was normalized to GAPDH and then normalized to WT SMCs. *P<.05; **P<.01, NOTCH1−/− SMCs versus WT SMCs. DAPI, 4′,6-diamidino-2-phenylindole; SOX10, SRY-related HMG-box 10; iPSCs, induced pluripotent stem cells; NCSCs, neural crest stem cells; TFAP2A, transcription factor AP-2 alpha; SMA, smooth muscle α-actin; SMC, smooth muscle cell; MYH11, myosin 11; CNN1, calponin 1; TAGLN, transgelin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: 13 One million iPSCs were electrotransfected with constructed 5 μ g of PX458/ NOTCH1 sgRNA using Lonza Human Stem Cell Nucleofector Kit 2 with program U-023 on a Nuclefector 2 device (Lonza Ltd, Basel, Switzerland).

Techniques: Knock-Out, Immunostaining, Real-time Polymerase Chain Reaction, Expressing, Western Blot

Discussion of the significance and the role of NOTCH1 in smooth muscle cell and endothelial cell differentiation from human iPSCs and future research direction. Video available at: http://www.jtcvsonline.org/article/S0022-5223(18)30714-1/fulltext.

Journal: The Journal of thoracic and cardiovascular surgery

Article Title: Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy

doi: 10.1016/j.jtcvs.2018.02.087

Figure Lengend Snippet: Discussion of the significance and the role of NOTCH1 in smooth muscle cell and endothelial cell differentiation from human iPSCs and future research direction. Video available at: http://www.jtcvsonline.org/article/S0022-5223(18)30714-1/fulltext.

Article Snippet: 13 One million iPSCs were electrotransfected with constructed 5 μ g of PX458/ NOTCH1 sgRNA using Lonza Human Stem Cell Nucleofector Kit 2 with program U-023 on a Nuclefector 2 device (Lonza Ltd, Basel, Switzerland).

Techniques: Cell Differentiation